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Applied and environmental microbiology. 2005-06-01; 71.6: 3342-7.

Genomic DNA amplification from a single bacterium

Raghunathan A, Ferguson HR, Bornarth CJ, Song W, Driscoll M, Lasken RS

PMID:

Abstract

Genomic DNA was amplified about 5 billion-fold from single, flow-sorted bacterial cells by the multiple displacement amplification (MDA) reaction, using phi 29 DNA polymerase. A 662-bp segment of the 16S rRNA gene could be accurately sequenced from the amplified DNA. MDA methods enable new strategies for studying non-culturable microorganisms.

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